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Vol 20, No 3 (2023)
- Year: 2023
- Articles: 7
- URL: https://cijournal.ru/1684-7849/issue/view/8466
- DOI: https://doi.org/10.17816/CI.2023203
Reviews
Oxidative stress as a marker of inflammation in cataracts
Abstract
Introduction. Oxidative stress is an important pathogenetic factor of degenerative eye diseases. Oxidative stress can damage tissues, leading to changes in their structure and function, increased vascular permeability, microvascular abnormalities and neovascularization. In turn, these changes can cause damage to the eye; denaturation of the crystalline lens.
The purpose of the study: according to modern literature, to study the role of oxidative stress and inflammation in the development of cataracts.
The molecular mechanisms of cell damage under oxidative stress and the pathogenesis of cataracts caused by oxidative stress have been studied.
Conclusion. Oxidative stress is an important pathogenetic mechanism of degenerative eye diseases. Oxidized phospholipids activate pro-inflammatory molecules and cause inflammation. Oxidative stress in the body is regulated by antioxidant mechanisms. An imbalance of antioxidants affects the lens and causes cataracts. The G/G genotype of SOD1-251 A/G polymorphism may lead to a higher risk of senile cataracts. The content of antioxidants depends on the density of the lens nuclei, on the type of cataract. Non-enzymatic antioxidants have a predominant effect in watery moisture, and enzymatic antioxidants in blood serum in patients with cataracts.
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Original Study Article
Prevalence of variants of the FAD-dependent amino oxidase gene in adolescents with internet addiction
Abstract
The aim of the research was to study the prevalence of rs1137070 MAOA polymorphism in Tuvan, Khakass and Russian adolescents, as well as the degree of their Internet addiction, assessed on the Chen scale. Materials and methods. The study included 559 adolescents of the Angara-Yenisei macroregion (Tuvinians, Khakas, Russians). The type of Internet addiction was assessed on the Chen scale, polymorphism was detected using real-time PCR. Results. It was revealed that the frequency of the TT rs1137070 genotype of the MAOA associated with low activity of the MAOA enzyme is significantly higher in Tuvan adolescents compared to Russians (31.1% vs. 21.2%, p=0.020). Pathological Internet addiction is more often observed among Khakas compared to Russian adolescents (26.4% vs. 15.5%, p=0.028). Thus, it is shown that the presence of a tendency to Internet addiction is potentially observed in carriers of the allele variant T rs1137070 MAOA, and Tuvans and Khakas, like other Asian populations, are more susceptible to the development of pathological dependencies, relative to Russians.
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Polymorphism of IL4 and IL13 anti-inflammatory cytokine genes in indigenous populations of the russian Arctic
Abstract
Living conditions in the Arctic zone are extremely unfavorable for living and contribute to the development of a number of diseases due to suppression of the functions of the immune system. Cytokines are one of the main mediators of the immune system; they are encoded by genes with a high degree of polymorphism. This study examined the distribution of genetic variants in the cytokine genes (rs2243250 IL4, rs1800925 IL13) among the Nenets, Dolgan-Nganasan and Slavs. It was previously established that these mutations are associated with the level of expression of these interleukins and their production, which leads to changes in the impairment of the immune response to the influence of the pathogen. The study showed the predominance of genotypes CT and TT of the rs2243250 IL4 polymorphism, and genotype CC of the rs1800925 IL13 polymorphism in the Nenets and Dolgan-Nganasan populations. The results obtained suggest that the indigenous inhabitants of the Russian Arctic have a genetically determined rapid immune response and protection against the development of allergic diseases compared to the Slavs.
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Clinical features and lymphocytes metabolic parameters of children 7-11 years with gallbladder dysfunction and with its association with giardiasis
Abstract
Purpose. Investigation of the clinical features and metabolic status of blood lymphocytes in children aged 7-11 years with gallbladder dysfunction depending on the presence or absence of giardiasis.
Materials and methods. Children aged 7–11 years with gallbladder dysfunction (GD) were examined in depending on the presence or absence of giardiasis. Enzyme activity in blood lymphocytes was determined by bioluminescent method.
Results. There are diarrheal disorders are more common in clinic dysfunction of gallbladder and flatulence – for gallbladder dysfunction in association with giardiasis. Enlargement of the liver and cystic positive symptoms are also characterized by dysfunction of gallbladder on a background of giardiasis. Significant changes in the NAD(P)-dependent dehydrogenases lymphocytes activity found in children with gallbladder dysfunction: reduced levels of substrate flux at the terminal glycolysis reactions and the tricarboxylic acid cycle, which determines the inhibition, respectively, anaerobic and aerobic respiration of cells.
Conclusion. Found that children with combined pathology had in lymphocytes, while maintaining the intensity of the terminal reactions of anaerobic glycolysis at the reference level reduced enzyme activity, determining the state of aerobic respiration. Independently from the presence or absence of giardiasis in children with gallbladder dysfunction reduced activity of the pentose phosphate cycle that characterizes the inhibition of the corresponding reactions of macromolecular synthesis.
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Dependence of hematological parameters on the microbiota of the nasal mucosa in chronic rhinitis
Abstract
Relevance. Chronic rhinitis is one of the most common otorhinolaryngological diseases. We assume that dysbiosis of the nasal mucosa depends on the phenotype of chronic rhinitis and affects hematological characteristics.
Aim. Study of the hematological parameters dependence from the microbiota of the nasal mucosa in patients with various forms of chronic rhinitis.
Material and methods. The examination groups included 45 patients with chronic allergic rhinitis (CAlR), 49 patients with chronic vasomotor rhinitis (CVR), 32 patients with chronic atrophic rhinitis (CAR), 39 patients with chronic infectious rhinitis (CIR) and 40 people from the control group. The subjects with chronic rhinitis, as well as the control group, underwent bacteriological examination of the nasal mucosa and assessment of hematological parameters. Statistical analysis was carried out using the Statistica 10 package.
Results. The microbiome of the nasal mucosa is characterized by 100% infection with infectious agents in CIR, while fungal flora was detected in 1/3 of cases. The microbiological features of the nasal mucosa in CVR corresponded to the control, in CAR rhinitis - predominantly the growth of Klebsiella spp., in CIR - Staphylococcus spp., Streptococcus spp., Neisseriae spp., Escherichia spp. Hematological features manifested themselves depending on the form of chronic rhinitis. The greatest number of changes in blood parameters is found in CIR and CAR, less in CAlR, no changes were detected in CVR. The greatest dysbiosis of the nasal mucosa in CIR, the abundant growth of microorganisms of the genus Klebsiella spp in CAR corresponds to the largest number of changes in hematological parameters, and CVR is characterized by the absence of changes in hematological parameters, despite the fact that the microbiological characteristics of the nasal mucosa corresponded to the control.
Conclusion. The microbiota of the nasal mucosa in healthy people and patients from the groups with CAlR, CVR and CAR is represented by a combination of coccal and rod-shaped flora, with the exception of CIR, in which representatives of the fungal flora are identified. Pathogenetically significant is the identification of representatives of Staphylococcus spp., Streptococcus spp., Neisseriae spp., Escherichia spp. in CIR, and Klebsiella spp. in CAR. Most hematological changes are detected in chronic atrophic and infectious rhinitis.
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Clinical and hematological characteristics of complicated and intact otitis media
Abstract
Purpose: to evaluate clinical and hematological features and identify patterns in hematological indices in patients with complicated and uncomplicated forms of otitis media.
Materials and methods: The study included 30 patients with otitis media aged from 18 to 70 years: 24 patients (80%) had an uncomplicated course, 6 (20%) had a complicated course (cholesteatomas, mastoiditis). The diagnosis was established taking into account the clinical recommendations of the Russian Ministry of Health (2022) on the basis of a comprehensive clinical and laboratory examination, otoscopy, and audiological studies.
Clinical, anamnestic and statistical methods were used. Calculation of hematological indices was carried out using a computer program. Statistical analysis was carried out using the Statistica 10 package. The critical level of statistical significance when testing scientific hypotheses was p < 0.05.
Results and conclusions. In the group of patients with complicated otitis media, a more vivid clinical picture and more pronounced changes in hematological parameters were observed. Noteworthy are the features of disturbances in the state of immune homeostasis: with complicated otitis, the ISLE indicator increased 3 times more often, which indicates the predominance of an immediate type reaction in the development of complications. The macrophage system index (MSI) was changed in uncomplicated otitis compared to complicated patients, which indicates a violation of cellular phagocytic protection in otitis media. In all cases, a violation of the effector and affector links of immunity (IAM) was revealed, with a predominance of the effector pathway. In 80% of cases of otitis, disturbances in the interaction of humoral and cellular immunity (LI) were observed.
The multidirectionality of the results obtained requires their further study in order to develop an algorithm for personalized diagnosis of patients with otitis media.
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Association of hematological parameters on polymorphisms of HFE gene (rs1800562, rs1800730, rs1799945) in non-alcoholic fatty liver disease
Abstract
Background. Non-alcoholic fatty liver disease and its associated metabolic syndrome are one of the most common chronic liver diseases among adults worldwide. One of the reasons associated with unfavorable course may be an abnormalities of iron metabolism associated with of fatty liver degeneration, caused by the presence of a certain polymorphic locus of the HFE gene.
Aim. Study the dependence of changes in hematological parameters on HFE gene polymorphisms among patients with non-alcoholic fatty liver disease with metabolic syndrome and normal body weight.
Materials and methods. The study included 173 patients, of which 85 people had NAFLD and normal body weight, 88 people had NAFLD and metabolic syndrome. All patients underwent genotyping of the rs1800562, rs1800730, rs1799945 polymorphisms of the HFE gene, as well as assessment of hematological parameters. Statistical analysis was performed out using the Statistica 10 package.
Results. Patients with NAFLD and normal body weight were characterized by the presence of the AT genotype of the rs1800730 polymorphism and the CG and GG genotypes of the rs1799945 polymorphism. In patients with NAFLD and metabolic syndrome, genotypes AA and TT of the rs1800730 polymorphism and CC of the rs1799945 polymorphism were more common.
Changes in hematological parameters were noted in both groups. In patients with normal body weight, an increase in ESR and monocytosis was observed. Patients with metabolic syndrome had anemia, increased ESR and pancytopenia.
Conclusion. Patients with NAFLD and normal body weight are characterized by the AT genotypes of the rs1800730 polymorphism and the CG and GG genotypes of the rs1799945 polymorphism. In patients with metabolic syndrome, variants of the AA polymorphism rs1800730 and CC polymorphism rs1799945 are more common. The rs1800562 polymorphism did not show significant differences in prevalence when compared between groups.
In patients with normal body weight, changes in hematological parameters are less pronounced, an increase in ESR and monocytosis is noted. In patients with metabolic syndrome, on the contrary, disturbances in hematological parameters are more pronounced; panleukopenia, increased ESR, and anemia are noted.
The changes we discovered may be evidence that in patients with NAFLD and normal body weight, steatosis and chronic inflammation predominated, and in patients with metabolic syndrome and NAFLD, steatohepatitis, which has a negative effect on organ systems. One of the risk factors for its development was the AA genotype of the rs800730 polymorphism and the CC genotype of the rs1799945 polymorphism.
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