Evaluation of physiological and immunological parameters in the offspring born from passively smoking rats treated with neuropeptides
- Authors: Smolyagin A.I.1, Simbirtsev A.S.2, Mikhailova I.V.1, Ermolina E.V.1, Isengulova A.A.1, Pushkareva L.A.1, Kuzmicheva N.А.1, Filippova Y.V.1, Chaynikova I.N.1, Miroshnichenko I.V.1
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Affiliations:
- Orenburg State Medical University
- State Research Institute of Highly Pure Biopreparations
- Issue: Vol 19, No 1-4 (2022)
- Pages: 75-79
- Section: Articles
- URL: https://cijournal.ru/1684-7849/article/view/623116
- DOI: https://doi.org/10.17816/CI2022221-4-12
- ID: 623116
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Abstract
The results of physiological and immunological studies of rat pups born from females who were exposed to passive smoking and exposure to tetrapeptide KK1 during pregnancy are presented. From the 1st to the 21st postnatal days, the somatic and sensorimotor development of offspring was studied using a set of tests developed at the P.K. Anokhin Institute of Normal Physiology. The level of cytokines IFN gamma and IL-6 produced by rat splenocytes was studied in culture fluid by the ELISA method. In baby rats born from passively smoking females, compared with animals born from non-smoking rats, a decrease in the weight of baby rats was found, as well as a delay in the timeliness of somatic and sensorimotor development. The introduction of the peptide KK 1 non-smoking females increases the index of timeliness of somatic and sensorimotor development.
The results obtained show an induced variant of secondary immunodeficiency in baby rats born from passively smoking rats during pregnancy. Administration of the KK1 peptide to non-smoking rats did not change the immunological parameters of the baby rats. On the contrary, the production of peptide KK1 by pregnant rats who smoked was accompanied by changes in the number of cells in lymphoid organs and the production of cytokines by splenocytes in the born rats.
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About the authors
Alexander I. Smolyagin
Orenburg State Medical University
Author for correspondence.
Email: probllab.orenburg@mail.ru
md, professor, honored worker of the higher school of the russian federation, professor of the department of clinical laboratory diagnostics
Russian Federation, 460000, Orenburg, Sovetskaya str., 6Andrey S. Simbirtsev
State Research Institute of Highly Pure Biopreparations
Email: cytokines@yandex.ru
corresponding member of the Russian academy of sciences, professor
Russian Federation, St.PetersburgIrina V. Mikhailova
Orenburg State Medical University
Email: cytokines@yandex.ru
doctor of biological sciences, associate professor, head of the department of pharmaceutical chemistry
Russian Federation, 460000, Orenburg, Sovetskaya str., 6Evgenia V. Ermolina
Orenburg State Medical University
Email: cytokines@yandex.ru
candidate of biological sciences, biologist of the immunological laboratory
Russian Federation, 460000, Orenburg, Sovetskaya str., 6Ainagul A. Isengulova
Orenburg State Medical University
Email: cytokines@yandex.ru
candidate of medical sciences, associate professor, associate professor of the department of normal physiology
Russian Federation, 460000, Orenburg, Sovetskaya str., 6Ludmila A. Pushkareva
Orenburg State Medical University
Email: cytokines@yandex.ru
postgraduate student of the department of normal physiology
Russian Federation, 460000, Orenburg, Sovetskaya str., 6Natalia А. Kuzmicheva
Orenburg State Medical University
Email: cytokines@yandex.ru
senior lecturer of the department of pharmaceutical chemistry
Russian Federation, 460000, Orenburg, Sovetskaya str., 6Yulia V. Filippova
Orenburg State Medical University
Email: cytokines@yandex.ru
candidate of medical sciences, associate professor of the department of pharmaceutical chemistry
Russian Federation, 460000, Orenburg, Sovetskaya str., 6Irina N. Chaynikova
Orenburg State Medical University
Email: cytokines@yandex.ru
md, professor of the department of normal physiology
Russian Federation, 460000, Orenburg, Sovetskaya str., 6Igor V. Miroshnichenko
Orenburg State Medical University
Email: cytokines@yandex.ru
professor, head of the department of normal physiology
Russian Federation, 460000, Orenburg, Sovetskaya str., 6References
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