Galectin-3 as a modulator of cytokine-mediated lymphocyte cooperation in vitro

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Аннотация

Aim of the study. To investigate the effect of recombinant galectin-3 on the cytokines secretion of various subpopulations of helper-lymphocytes (Th1, Th2, Th17, Treg) in culture in vitro. Material and methods. The material for the study was peripheral blood from healthy people (n=17), from which lymphocytes were isolated by gradient centrifugation. Lymphocytes were cultured for 72 hours in a CO2-incubator with recombinant galectin-3 and activating antibodies (antiCD3/antiCD28). The concentration of cytokines (IL-10, IL-13, IL-17A, IL-22, IFNγ, TNFα, TGFβ1) in the supernatants of lymphocyte cultures was determined by enzyme immunoassay. Results. Recombinant galectin-3 in vitro enhanced the secretion of IL-17A, IL-22 by lymphocytes, acting at a dose of 0.5 μg/ml; IL-13, TNFα and IFNγ at doses of 0.5 μg/ml and 1 μ/ml (more pronounced when acting at a dose of 0.5 μg/ml) and inhibited the production of IL-17, IL-22 at a dose of 1 μg/ml and IL-10, TGFβ1 when testing both concentrations. Conclusion: Galectin-3 has a dose-dependent modulating effect on the cytokine-producing function of healthy donors lymphocytes in vitro. Functional imbalance in blood lymphocytes under the action of recombinant galectin-3 is manifested by induction of pro-inflammatory (IFNγ, IL-17, IL-22, TNFα) and anti-inflammatory (interleukin-13) cytokines secretion, against the background of oppression of the suppressor cytokines (IL-10 and TGFβ1) production. A detailed study of the immunotropic effects of galectin-3 in relation to individual lymphocytes subpopulations is relevant from the view point of development new approaches to the treatment of tumor and autoimmune diseases accompanied by excessive production of this lectin.

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Olga Vasil’eva

Siberian State Medical University, SSMU

Хат алмасуға жауапты Автор.
Email: vasiljeva-24@yandex.ru

candidate of medical sciences, associate professor of the department of pathophysiology, associate professor of the department of biochemistry and molecular biology with a course of clinical laboratory diagnostics

Ресей, 634050, Tomsk, Moskovsky tract, 2

T. Prokhorenko

Tomsk Regional Blood Center

Email: cytokines@yandex.ru

candidate of medical sciences, doctor of clinical laboratory diagnostics

Ресей, Tomsk

Y. Kolobovnikova

Siberian State Medical University, SSMU

Email: cytokines@yandex.ru

doctor of medical sciences, associate professor, professor of the department of pathophysiology, acting head of the department of normal physiology

Ресей, 634050, Tomsk, Moskovsky tract, 2

V. Poletika

Siberian State Medical University, SSMU

Email: cytokines@yandex.ru

candidate of medical sciences, associate professor of the department of pathophysiology

Ресей, 634050, Tomsk, Moskovsky tract, 2

O. Urazova

Siberian State Medical University, SSMU

Email: cytokines@yandex.ru

doctor of medical sciences, professor, corresponding member of the Russian academy of sciences, head of the department of pathophysiology

Ресей, 634050, Tomsk, Moskovsky tract, 2

T. Kononova

Siberian State Medical University, SSMU

Email: cytokines@yandex.ru

candidate of medical sciences, associate professor of the department of pathophysiology

Ресей, 634050, Tomsk, Moskovsky tract, 2

E. Churina

Siberian State Medical University, SSMU

Email: cytokines@yandex.ru

doctor of medical sciences, professor of the department of pathophysiology

Ресей, 634050, Tomsk, Moskovsky tract, 2

G. Reynhardt

Siberian State Medical University, SSMU

Email: cytokines@yandex.ru

postgraduate student of the department of pathophysiology

Ресей, 634050, Tomsk, Moskovsky tract, 2

A. Kurnosenko

Siberian State Medical University, SSMU

Email: cytokines@yandex.ru

postgraduate student of the department of pathophysiology

Ресей, 634050, Tomsk, Moskovsky tract, 2

Әдебиет тізімі

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© Vasil’eva O.A., Prokhorenko T.S., Kolobovnikova Y.V., Poletika V.S., Urazova O.I., Kononova T.E., Churina E.G., Reynhardt G.V., Kurnosenko A.V., 2023

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