Effects of VDBP rs4588 and rs7041 gene polymorphism on vitamin D status in obese individuals
- Authors: Shnar V.A.1, Paskidov D.V.1, Bograya M.M.1, Voronova S.S.1, Vulf M.A.1, Gazatova N.D.1, Mikhailova L.V.1, Litvinova L.S.1
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Affiliations:
- Immanuel Kant Baltic Federal University
- Issue: Vol 21, No 1 (2024)
- Pages: 46-53
- Section: Short communications
- URL: https://cijournal.ru/1684-7849/article/view/634869
- DOI: https://doi.org/10.17816/CI634869
- ID: 634869
Cite item
Abstract
Vitamin D plays a major role in bone metabolism regulating body phosphorus and calcium metabolism. Vitamin D binding protein (VDBP) binds to vitamin D metabolites and transports them to target tissues. It is hypothesized that polymorphisms in VDBP affect the absorption of vitamin D in the body. However, the relationship between VDBP polymorphisms and vitamin D levels in individuals with obesity has yet to be sufficiently investigated.
This work aimed to identify the relationship between vitamin D [25(OH)D3] blood levels in obese and non-obese individuals and the rs4588 and rs7041 polymorphisms in VDBP.
The study included a total of 80 participants from the Kaliningrad region. The sample was stratified based on vitamin D status (normal, insufficiency, deficiency, and acute deficiency) and body mass index (BMI). Vitamin D blood levels were determined using enzyme immunoassay. Genomic DNA was extracted from whole blood samples. VDBP polymorphisms were identified using HaeIII and ErhI restriction endonucleases.
No significant differences in vitamin D blood levels between obese and non-obese individuals were revealed. The rs4588 C > A genotypes were associated with no significant differences in vitamin D levels between individuals with different body mass index (CC, p=0.147; CA, p=0.646; AA, insufficient data). However, for the VDBP rs4588 polymorphism, a significant difference was demonstrated in allele frequency between the groups with different vitamin D levels, i.e., normal vitamin D level group vs. insufficiency group (p=0.009) and normal vitamin D level group vs. acute deficiency group (p <0.0001).
The analysis of the individuals with the VDBP rs7041 T > G polymorphism demonstrated that there were no significant differences in the vitamin D levels between the BMI groups with specific genotypes (TG, p=0.502; GG, p=0.283; TT, insufficient data). However, the control and acute deficiency groups differed significantly (p <0.0001) in allele frequencies of individuals with the rs7041 polymorphism.
Vitamin D blood levels in obese patients were comparable to those of non-obese individuals. VDBP rs7041 and rs4588 polymorphisms do not correlate with variations in vitamin D blood levels among individuals with different BMI. However, the VDBP rs4588 A polymorphism is associated with low vitamin D levels, while the VDBP rs4588 G polymorphism is more common among individuals with normal vitamin D blood levels.
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About the authors
Varvara A. Shnar
Immanuel Kant Baltic Federal University
Email: shnar.v@yandex.ru
ORCID iD: 0009-0003-4255-938X
SPIN-code: 6792-7122
Russian Federation, Kaliningrad
Dimitri V. Paskidov
Immanuel Kant Baltic Federal University
Email: dima_2121@icloud.com
ORCID iD: 0009-0008-0241-8036
SPIN-code: 8562-7200
Russian Federation, Kaliningrad
Maria M. Bograya
Immanuel Kant Baltic Federal University
Author for correspondence.
Email: mbograya@mail.ru
ORCID iD: 0000-0002-4543-7850
SPIN-code: 2488-5439
Russian Federation, Kaliningrad
Sophia S. Voronova
Immanuel Kant Baltic Federal University
Email: nraven352@icloud.com
ORCID iD: 0000-0002-3358-6226
SPIN-code: 1347-5285
Scopus Author ID: 58519735600
Russian Federation, Kaliningrad
Maria A. Vulf
Immanuel Kant Baltic Federal University
Email: mary-jean@yandex.ru
ORCID iD: 0000-0002-4989-045X
SPIN-code: 1525-4854
Cand. Sci. (Biology)
Russian Federation, KaliningradNatalia D. Gazatova
Immanuel Kant Baltic Federal University
Email: NGazatova@kantiana.ru
ORCID iD: 0000-0002-4646-3436
SPIN-code: 1174-7330
Cand. Sci. (Biology)
Russian Federation, KaliningradLarisa V. Mikhailova
Immanuel Kant Baltic Federal University
Email: mihalysa@mail.ru
ORCID iD: 0000-0001-5070-5955
SPIN-code: 4991-9875
MD, Cand. Sci. (Medicine), Assistant Professor
Russian Federation, KaliningradLarisa S. Litvinova
Immanuel Kant Baltic Federal University
Email: larisalitvinova@yandex.ru
ORCID iD: 0000-0001-5231-6910
SPIN-code: 6703-3412
MD, Dr. Sci. (Medicine), Assistant Professor
Russian Federation, KaliningradReferences
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