Effects of VDBP rs4588 and rs7041 gene polymorphism on vitamin D status in obese individuals

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Abstract

Vitamin D plays a major role in bone metabolism regulating body phosphorus and calcium metabolism. Vitamin D binding protein (VDBP) binds to vitamin D metabolites and transports them to target tissues. It is hypothesized that polymorphisms in VDBP affect the absorption of vitamin D in the body. However, the relationship between VDBP polymorphisms and vitamin D levels in individuals with obesity has yet to be sufficiently investigated.

This work aimed to identify the relationship between vitamin D [25(OH)D3] blood levels in obese and non-obese individuals and the rs4588 and rs7041 polymorphisms in VDBP.

The study included a total of 80 participants from the Kaliningrad region. The sample was stratified based on vitamin D status (normal, insufficiency, deficiency, and acute deficiency) and body mass index (BMI). Vitamin D blood levels were determined using enzyme immunoassay. Genomic DNA was extracted from whole blood samples. VDBP polymorphisms were identified using HaeIII and ErhI restriction endonucleases.

No significant differences in vitamin D blood levels between obese and non-obese individuals were revealed. The rs4588 C > A genotypes were associated with no significant differences in vitamin D levels between individuals with different body mass index (CC, p=0.147; CA, p=0.646; AA, insufficient data). However, for the VDBP rs4588 polymorphism, a significant difference was demonstrated in allele frequency between the groups with different vitamin D levels, i.e., normal vitamin D level group vs. insufficiency group (p=0.009) and normal vitamin D level group vs. acute deficiency group (p <0.0001).

The analysis of the individuals with the VDBP rs7041 T > G polymorphism demonstrated that there were no significant differences in the vitamin D levels between the BMI groups with specific genotypes (TG, p=0.502; GG, p=0.283; TT, insufficient data). However, the control and acute deficiency groups differed significantly (p <0.0001) in allele frequencies of individuals with the rs7041 polymorphism.

Vitamin D blood levels in obese patients were comparable to those of non-obese individuals. VDBP rs7041 and rs4588 polymorphisms do not correlate with variations in vitamin D blood levels among individuals with different BMI. However, the VDBP rs4588 A polymorphism is associated with low vitamin D levels, while the VDBP rs4588 G polymorphism is more common among individuals with normal vitamin D blood levels.

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About the authors

Varvara A. Shnar

Immanuel Kant Baltic Federal University

Email: shnar.v@yandex.ru
ORCID iD: 0009-0003-4255-938X
SPIN-code: 6792-7122
Russian Federation, Kaliningrad

Dimitri V. Paskidov

Immanuel Kant Baltic Federal University

Email: dima_2121@icloud.com
ORCID iD: 0009-0008-0241-8036
SPIN-code: 8562-7200
Russian Federation, Kaliningrad

Maria M. Bograya

Immanuel Kant Baltic Federal University

Author for correspondence.
Email: mbograya@mail.ru
ORCID iD: 0000-0002-4543-7850
SPIN-code: 2488-5439
Russian Federation, Kaliningrad

Sophia S. Voronova

Immanuel Kant Baltic Federal University

Email: nraven352@icloud.com
ORCID iD: 0000-0002-3358-6226
SPIN-code: 1347-5285
Scopus Author ID: 58519735600
Russian Federation, Kaliningrad

Maria A. Vulf

Immanuel Kant Baltic Federal University

Email: mary-jean@yandex.ru
ORCID iD: 0000-0002-4989-045X
SPIN-code: 1525-4854

Cand. Sci. (Biology)

Russian Federation, Kaliningrad

Natalia D. Gazatova

Immanuel Kant Baltic Federal University

Email: NGazatova@kantiana.ru
ORCID iD: 0000-0002-4646-3436
SPIN-code: 1174-7330

Cand. Sci. (Biology)

Russian Federation, Kaliningrad

Larisa V. Mikhailova

Immanuel Kant Baltic Federal University

Email: mihalysa@mail.ru
ORCID iD: 0000-0001-5070-5955
SPIN-code: 4991-9875

MD, Cand. Sci. (Medicine), Assistant Professor

Russian Federation, Kaliningrad

Larisa S. Litvinova

Immanuel Kant Baltic Federal University

Email: larisalitvinova@yandex.ru
ORCID iD: 0000-0001-5231-6910
SPIN-code: 6703-3412

MD, Dr. Sci. (Medicine), Assistant Professor

Russian Federation, Kaliningrad

References

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Supplementary files

Supplementary Files
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2. Fig. 1. The rs4588 polymorphism: a — genotype-specific vitamin D levels; b — vitamin D levels in the obese and non-obese individuals of specified genotypes; c — allele frequency in vitamin D level groups; d — allele frequency in obese and non-obese groups. Only statistically significant p-values are indicated in the figures.

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3. Fig. 2. The rs7041 polymorphism: a — genotype-specific vitamin D levels; b — vitamin D levels in the obese and non-obese individuals of specified genotypes; c — allele frequency in vitamin D level groups; d — allele frequency in obese and non-obese groups. Only statistically significant p-values are indicated in the figures.

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Copyright (c) 2024 Shnar V.A., Paskidov D.V., Bograya M.M., Voronova S.S., Vulf M.A., Gazatova N.D., Mikhailova L.V., Litvinova L.S.

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