Features of phagocytic and chemiluminescent activity of neutrophils when exposed to polyprenols in vitro
- Authors: Savchenko A.A.1, Belenyuk V.D.1, Gvozdev I.I.1, Borisov A.G.1
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Affiliations:
- Krasnoyarsk Scientific Center of the Siberian Branch of the Russian Academy of Sciences of the «Research Institute of Medical Problems of the North»
- Issue: Vol 20, No 1 (2023)
- Pages: 47-53
- Section: Original Study Article
- URL: https://cijournal.ru/1684-7849/article/view/623779
- DOI: https://doi.org/10.17816/CI2023231-7
- ID: 623779
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Abstract
Aim: Studying of the features of phagocytic activity and the state of the respiratory burst of neutrophils under the influence of polyprenols in vitro.
Materials and methods. The study involved 30 relatively healthy donors aged 23-59 years from whose peripheral blood neutrophilic granulocytes were isolated to determine phagocytic and chemiluminescent activity. The isolated cells were incubated for 30 minutes with polyprenols in vitro. The level of phagocytosis of immature and mature neutrophils was determined by flow cytometry using FITC-labeled staphylococcal protein A. The state of the respiratory burst was assessed using chemiluminescent analysis.
Results. It was found that the levels of phagocytic index (PI) and number (PN) of mature neutrophils were reduced after incubation with polyprenols relative to initial and control values. The values of PI and PN of immature neutrophils under the influence of polyprenols and during control incubation were decreased relative to the initial values. The intensity maximum and area under the curve of spontaneous and zymosan-induced lucigenin- and luminol-dependent chemiluminescence of the neutrophils were reduced after incubation with polyprenols.
Conclusion. The effect of polyprenols on neutrophilic granulocytes in vitro leads to a decrease in activity in respiratory burst cells, which is realized in blocking by polyprenols the interaction of primary and secondary ROS with chemiluminescent indicators by the mechanism of primary antioxidants. The binding of ROS by polyprenols also manifests itself in a decrease in the phagocytic activity of mature neutrophils while maintaining the phagocytic activity of immature cells at the level of control incubation. In general, polyprenols exhibit anti-inflammatory activity when exposed in vitro to neutrophilic granulocytes.
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About the authors
Andrey A. Savchenko
Krasnoyarsk Scientific Center of the Siberian Branch of the Russian Academy of Sciences of the «Research Institute of Medical Problems of the North»
Email: 2410454@mail.ru
ORCID iD: 0000-0001-5829-672X
SPIN-code: 3132-8260
д.м.н., профессор, заведующий лабораторией клеточно-молекулярной физиологии и патологии
Russian Federation, KrasnoyarskVasily D. Belenyuk
Krasnoyarsk Scientific Center of the Siberian Branch of the Russian Academy of Sciences of the «Research Institute of Medical Problems of the North»
Email: 2410454@mail.ru
ORCID iD: 0000-0003-2848-0846
SPIN-code: 6195-6630
младший научный сотрудник лаборатории клеточно-молекулярной физиологии и патологии
Russian Federation, KrasnoyarskIvan I. Gvozdev
Krasnoyarsk Scientific Center of the Siberian Branch of the Russian Academy of Sciences of the «Research Institute of Medical Problems of the North»
Email: 2410454@mail.ru
ORCID iD: 0000-0002-1041-9871
SPIN-code: 6203-4651
младший научный сотрудник лаборатории клеточно-молекулярной физиологии и патологии
Russian Federation, KrasnoyarskAlexander G. Borisov
Krasnoyarsk Scientific Center of the Siberian Branch of the Russian Academy of Sciences of the «Research Institute of Medical Problems of the North»
Author for correspondence.
Email: 2410454@mail.ru
ORCID iD: 0000-0002-9026-2615
SPIN-code: 9570-2254
к.м.н., ведущий научный сотрудник лаборатории клеточно-молекулярной физиологии и патологии
Russian Federation, KrasnoyarskReferences
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