Ascorbate biosynthesis and recycling genes are involved in the responses of garlic Allium sativum L. plants to Fusarium proliferatum infection

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Abstract

The expression profile of key genes of ascorbate biosynthesis (VTC2, GPP, GalDH, GalLDH) and recycling (MDHAR1, MDHAR4, MDHAR5) was determined in response to infection with the fungal pathogen Fusarium proliferatum in garlic cultivars related (Podnebesny) and sensitive (Dubkovsky) to Fusarium rot. It was found that differences in resistance to Fusarium lead to discrepancies in the dynamics and expression of individual genes of the ascorbate pathway, as well as in ascorbate content. It was shown that in response to infection, the expression level of the MDHAR4 gene increases in the resistant cultivar and decreases in the Fusarium-sensitive accession. As infection progresses, the expression levels of the VTC2 and GalLDH genes increase significantly (higher in the cv. Dubkovsky than in the cv. Podnebesny). In both cultivars, the ascorbate content increases (1.5 times higher in the cv. Dubkovsky than in the cv. Podnebesny).

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About the authors

A. V. Shchennikova

Institute of Bioengineering, Federal Research Center “Fundamentals of Biotechnology” of the Russian Academy of Sciences

Email: michel7753@mail.ru
Russian Federation, Moscow

E. Z. Kochieva

Institute of Bioengineering, Federal Research Center “Fundamentals of Biotechnology” of the Russian Academy of Sciences

Email: michel7753@mail.ru
Russian Federation, Moscow

M. A. Filyushin

Institute of Bioengineering, Federal Research Center “Fundamentals of Biotechnology” of the Russian Academy of Sciences

Author for correspondence.
Email: michel7753@mail.ru
Russian Federation, Moscow

References

  1. Smirnoff N. // Free Radical Biology and Medicine. 2018. V. 22. P. 116–129.
  2. Gill S.S., Tuteja N. // Plant Physiol. Biochem. 2010. V. 48. P. 909.
  3. Apel K, Hirt H. // Annu. Rev. Plant Biol. 2004. V. 55. P. 373–399.
  4. Kuźniak E., Kopczewski T., Chojak-Koźniewska J. // In: Hossain, M., et al. (eds) Ascorbic Acid in Plant Growth, Development and Stress Tolerance. Springer. 2017.
  5. Zurbriggen M.D., Carrillo N., Hajirezaei M.R. // Plant Signal Behav. 2010. V. 5(4). P. 393–396.
  6. Barth C., Moeder W., Klessig D.F., et al. // Plant Physiol. 2004. V. 134(4). P. 1784–1792.
  7. Abou-Attia M.A., Wang X., Nashaat Al-Attala M., et al. // Physiol. Plant. 2016. V. 156(3). P. 262–277.
  8. Broad R.C., Bonneau J.P., Hellens R.P., et al. // Int. J. Mol. Sci. 2020. V. 21. 1790.
  9. Ali B., Pantha S., Acharya R., et al. // J. Plant Physiol. 2019. V. 240. 152998.
  10. Anisimova O. K., Shchennikova A. V., Kochieva E.Z., et al. // Russian Journal of Genetics. 2022. V. 58(7). P. 773–782.
  11. Kuzniak E., Skłodowska M. // J. Exp. Bot. 2004. V. 55. P. 605–612.
  12. Anisimova O.K., Seredin T.M., Shchennikova A.V., et al. // Russian Journal of Plant Physiology. 2021. V. 68. P. 85–93.

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2. Fig. 1. a – Change in the expression levels of genes for ascorbate biosynthesis and recycling in the roots of garlic varieties Podnebesny and Dubkovsky 24 and 96 hours after infection with the pathogenic fungus Fusarium proliferatum. Primers: AsVTC2 (5´-ggtgtcaagcgtgtgtatctg-3´; 5´-ttcccaaacagcgggattgac-3´), AsGPP (5´-ggtcacagaaactgataaagcatg-3´; 5´-catcagtaagtagtagcagtgcc-3´), AsGalDH (5´-cctctgtccgcaaggctttt-3´; 5´-ctaccacacttggtggacac-3´), AsGalLDH (5´-tggactgctaggagcaagagt-3´; 5´-gccttgcgttaggcgtcga-3´), AsMDHAR1 (5´-tttgaaccctggcgagcttg-3´; 5´-ctggcagtaagcgttctcca-3´), AsMDHAR4 (5´-cgcaggttatgcagctcttg-3´; 5´-cgcctacgcaagtatgaaatgc-3´), AsMDHAR5 (5´-ggggctcgcatagataagttga-3´; 5´-tcccacggacttattcagcc-3´). b – Gene expression data (A), normalized to control. The expression level values ​​in the control at each point (24 and 96 h) are taken as 1; the expression level values ​​in the experimental (infected) samples are recalculated proportionally to the control. The color scale from blue to red corresponds to the dynamics of gene expression from decrease to increase (vs control). hpi – hours after infection

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3. Fig. 2. Changes in the AK content in the roots of garlic varieties Podnebesny and Dubkovsky 24 and 96 hours after infection with the pathogenic fungus Fusarium proliferatum. hpi – hours after infection

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