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Development and evaluation of the effectiveness of a multivalent DNA vaccine against brucellosis
- Authors: Khodarovich Y.M.1, Elshazly M.A.2, Marzanova S.N.3, Magomedova S.3, Shell W.S.2, Nassif S.A.2, Devrishov D.A.3, Deyev S.M.1
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Affiliations:
- Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
- Central Laboratory for Evaluation of Veterinary Biologics (CLEVB)-Agriculture Research Center (ARC)
- Federal State Educational Institution of Higher Professional Education Moscow State Academy of Veterinary Medicine and Biotechnology named after K.I. Skryabin
- Issue: Vol 520, No 1 (2025)
- Pages: 31-37
- Section: Articles
- URL: https://cijournal.ru/2686-7389/article/view/682025
- DOI: https://doi.org/10.31857/S2686738925010062
- EDN: https://elibrary.ru/tcxfpl
- ID: 682025
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Abstract
Vaccination against brucellosis is currently carried out using attenuated strains of live brucellae, which causes serious side effects in animals and is of little use for human vaccination. In order to develop a safer vaccine against brucellosis, we created plasmid DNA capable of expressing six brucellae proteins in mammalian cells. Linear polyethyleneimine was used to increase the efficiency of DNA delivery to cells. Data on optimization of the conditions for the formation of DNA-polyethyleneimine complexes are presented. It is shown that a single intramuscular or subcutaneous injection of plasmid DNA complexes with polyethyleneimine at a low dose (20 μg/mouse) has a reliable protective effect against subsequent infection of mice with a test strain of brucellae.
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About the authors
Y. M. Khodarovich
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Author for correspondence.
Email: khodarovich@mail.ru
Russian Federation, Moscow
M. A. Elshazly
Central Laboratory for Evaluation of Veterinary Biologics (CLEVB)-Agriculture Research Center (ARC)
Email: khodarovich@mail.ru
Egypt, Cairo
S. N. Marzanova
Federal State Educational Institution of Higher Professional Education Moscow State Academy of Veterinary Medicine and Biotechnology named after K.I. Skryabin
Email: khodarovich@mail.ru
Russian Federation, Moscow
S. Magomedova
Federal State Educational Institution of Higher Professional Education Moscow State Academy of Veterinary Medicine and Biotechnology named after K.I. Skryabin
Email: khodarovich@mail.ru
Russian Federation, Moscow
W. S. Shell
Central Laboratory for Evaluation of Veterinary Biologics (CLEVB)-Agriculture Research Center (ARC)
Email: khodarovich@mail.ru
Egypt, Cairo
S. A. Nassif
Central Laboratory for Evaluation of Veterinary Biologics (CLEVB)-Agriculture Research Center (ARC)
Email: khodarovich@mail.ru
Egypt, Cairo
D. A. Devrishov
Federal State Educational Institution of Higher Professional Education Moscow State Academy of Veterinary Medicine and Biotechnology named after K.I. Skryabin
Email: khodarovich@mail.ru
Corresponding Member of the RAS
Russian Federation, MoscowS. M. Deyev
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: khodarovich@mail.ru
Academician of the RAS
Russian Federation, MoscowReferences
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