Analysis of natural antibodies during the development of phantom pain syndrome

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Abstract

We determined natural antibodies (e-Abs) to the regulators of the main systems of biochemical homeostasis: β-endorphin, serotonin, dopamine, histamine, orphanin, angiotensin, GABA, glutamate, bradykinin, vasopressin, thrombin, α-2-macroglobulin in individuals with phantom pain syndrome (FBS), resulting from amputation after injury. It has been established that each patient has an individual immunoprofile, but for all of them there is a significant increase in the level of antibodies to serotonin, histamine and angiotensin, which reflect the chronicity of the pain syndrome and do not depend on the self-assessment of the severity of FBS. Determining the role of regulators of biochemical homeostasis in the development of phantom pain showed that with high, moderate and weak severity of FBS, the biogenic amine and angiotensinergic systems are activated. A decrease in FBS intensity normalizes deviations in all immunological parameters. The level of e-Abs for the pain (β-endorphin) and analgesic (orphanin) systems are significant only at low FBS. Monitoring the individual profile of e-Abs to endogenous regulators allows us to obtain an objective picture of the pain status of the patient’s body.

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About the authors

M. А. Myagkova

Institute of Physiologically Active Substances Federal Research Center for Problems of Chemical Physics and Medical Chemistry Russian Academy of Sciences

Email: dianark@mail.ru
Russian Federation, Chernogolovka, Moscow region

S. N. Petrochenko

Institute of Physiologically Active Substances Federal Research Center for Problems of Chemical Physics and Medical Chemistry Russian Academy of Sciences

Author for correspondence.
Email: dianark@mail.ru
Russian Federation, Chernogolovka, Moscow region

Z. V. Bobrova

Institute of Physiologically Active Substances Federal Research Center for Problems of Chemical Physics and Medical Chemistry Russian Academy of Sciences

Email: dianark@mail.ru
Russian Federation, Chernogolovka, Moscow region

E. А. Orlova

Institute of Physiologically Active Substances Federal Research Center for Problems of Chemical Physics and Medical Chemistry Russian Academy of Sciences

Email: dianark@mail.ru
Russian Federation, Chernogolovka, Moscow region

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Supplementary files

Supplementary Files
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2. Fig. 1. The results of comparing the level of e-At to endogenous regulators, for the group of examined patients No. 1-9. Along the abscissa axis: the value of KOD450 in ELISA for e-At to the analyzed endogenous regulators of each patient. Below is a list of analyzed endogenous regulators highlighted in color. The result of each examined patient is presented in accordance with the selected color for the antigen, as the value of KOD450 in ELISA. On the ordinate axis: the numbers of the examined patients.

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Presented by Academician of the RAS S.O. Bachyurin


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