Identification of an Exosome-relevant SNHG6-hsa-miR-429- CHRDL1/CCNA2 Axis for Lung Adenocarcinoma Prognosis Evaluation
- Autores: Yuan Y.1, Li Q.1, Chen F.1, Zhao Y.2, Ma J.1, Fang S.1
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Afiliações:
- Department of Respiration, Nanjing First Hospital
- Shenzhen Engineering Center for Translational Medicine of Precision Cancer Immunodiagnosis and Therapy, YuceBio Technology Co., Ltd, Regional marketing department
- Edição: Volume 31, Nº 28 (2024)
- Páginas: 4549-4561
- Seção: Anti-Infectives and Infectious Diseases
- URL: https://cijournal.ru/0929-8673/article/view/645234
- DOI: https://doi.org/10.2174/0109298673280925231122104717
- ID: 645234
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Resumo
Aims:To explore an exosome-relevant molecular classification in lung adenocarcinoma (LUAD).
Background:Exosome genes or relevant non-coding RNAs are regulators of cancer treatment and prognosis, but their function in LUAD has not yet been determined.
Objective:Unraveling a molecular classification applying exosome-related RNA networks for LUAD prognosis evaluation.
Methods:MicroRNA sequencing data (miRNAs-seq) and RNA sequencing data (RNA- seq) were derived from The Cancer Genome Atlas (TCGA). The ConsensusCluster- Plus package was used for molecular typing in LUAD based on 121 Exosome-related genes. Then, a limma package was conducted to explore differentially expressed mRNAs (DEmRNAs), differentially expressed miRNAs (DEmiRNAs) and differentially expressed lncRNAs (DElncRNAs) in molecular typing for constructing an Exosome-driven competing endogenous RNA network (ceRNA). Dominant miRNAs, as well as target mRNAs, were identified by COX modeling and Kaplan-Meier survival analysis.
Results:Two Exosome-associated molecular clusters classified in LUAD. The C2 cluster favored high clinicopathology and showed a trend toward poor prognosis. 29 lncRNA- miRNA and 12 miRNA-mRNA interaction pairs were identified. The hsa-miR-429 was the pivotal miRNA in the network that affected the prognosis of LUAD. According to the interaction relationship and LUAD prognostic role, SNHG6-hsa- miR-429-CHRDL1/CCNA2 was identified. SNHG6-hsa-miR-429-CHRDL1 exerts oncogenic effects, and SNHG6-hsa-miR-429- CCNA2 exerts pro-oncogenic effects.
Conclusion:Overall, our study identified an Exosome-driven ceRNA network in LUAD, and the SNHG6-hsa-miR-429-CHRDL1/CCNA2 axis could be a new therapeutic target for LUAD and our study provides new insights into the molecular mechanisms of LUAD.
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Sobre autores
Yuan Yuan
Department of Respiration, Nanjing First Hospital
Email: info@benthamscience.net
Qian Li
Department of Respiration, Nanjing First Hospital
Email: info@benthamscience.net
Feifei Chen
Department of Respiration, Nanjing First Hospital
Email: info@benthamscience.net
Yujie Zhao
Shenzhen Engineering Center for Translational Medicine of Precision Cancer Immunodiagnosis and Therapy, YuceBio Technology Co., Ltd, Regional marketing department
Email: info@benthamscience.net
Jiyong Ma
Department of Respiration, Nanjing First Hospital
Autor responsável pela correspondência
Email: info@benthamscience.net
Surong Fang
Department of Respiration, Nanjing First Hospital
Autor responsável pela correspondência
Email: info@benthamscience.net
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