Structure and function of the transglutaminase cluster in the basal metazoan Halisarca dujardinii (sponge)

Мұқаба

Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Тек жазылушылар үшін

Аннотация

Transglutaminases are enzymes that carry out post-translational modifications of proteins and participate in the regulation of their activities. Here, we show for the first time that the transglutaminase genes in the basal metazoan, sea sponge Halisarca dujardinii, are organized in a cluster, similar to mammalian transglutaminases. The regulatory regions of six transglutaminase genes and their differential expression in the course of H. dujardinii life cycle suggest independent regulation of these genes. The decrease in transglutaminase activities by cystamine facilitates restoration of the sponge multicellular structures after its mechanical dissociation. For the first time we observed that this decrease in transglutaminase activities was accompanied by generation of the reactive oxygen species in the cells of a basal metazoan. The study of transglutaminases in the basal metazoans and other sea-dwelling organisms might provide better understanding of evolution and specific functions of these enzymes in higher animals.

Толық мәтін

Рұқсат жабық

Авторлар туралы

A. Finoshin

Koltzov Institute of Developmental Biology, Russian Academy of Sciences

Хат алмасуға жауапты Автор.
Email: alexcolton@yandex.ru
Ресей, Moscow, 119334

O. Kravchuk

Koltzov Institute of Developmental Biology, Russian Academy of Sciences

Email: alexcolton@yandex.ru
Ресей, Moscow, 119334

K. Mikhailov

Lomonosov Moscow State University; Kharkevich Institute for Information Transmission Problems, Russian Academy of Sciences

Email: alexcolton@yandex.ru

Belozersky Institute of Physical and Chemical Biology

Ресей, Moscow, 119992; Moscow, 127051

R. Ziganshin

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Email: alexcolton@yandex.ru
Ресей, Moscow, 117997

K. Adameyko

Koltzov Institute of Developmental Biology, Russian Academy of Sciences

Email: alexcolton@yandex.ru
Ресей, Moscow, 119334

V. Mikhailov

Koltzov Institute of Developmental Biology, Russian Academy of Sciences

Email: alexcolton@yandex.ru
Ресей, Moscow, 119334

Yu. Lyupina

Koltzov Institute of Developmental Biology, Russian Academy of Sciences

Email: alexcolton@yandex.ru
Ресей, Moscow, 119334

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2. Fig. 1. Phylogenetic tree of animal TGM. Numbers on tree nodes correspond to node support estimates using the ultrafast bootstrap method UFBoot with 1000 replicates.

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3. Fig. 2. TGM genes of the sponge H. dujardinii (a) and humans (б). The numbers indicate the distances between genes in nucleotide pairs. The 5´ region of the H. dujardinii sponge genes shows regulatory sequences: TATA box; HRE – hypoxia response element; AP1, SP1, CREB/AP1 – binding sites with the corresponding transcription factors.

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4. Fig. 3. Expression of TGM genes during dissociation/reaggregation of the sponge H. dujardinii in different periods of the annual cycle. The left panel shows the gene expression level in the sponge body tissue in different periods of the annual cycle. Blue, light green, pink, and orange colors indicate winter, spring, summer, and autumn, respectively. *Statistically significant differences in TGM gene expression in the sponge body tissue in different seasons. The right panel shows the change in expression in dissociated cells and cell aggregates compared to the intact sponge. Large and small dots indicate dissociated cells and aggregates. Only statistically significant differences (FDR < 0.001, see Experimental Section) are shown on the heat map on the right.

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5. Fig. 4. Alignment of the amino acid sequences of the catalytic sites of H. dujardinii and human TGM. Arrows and boxes highlight the catalytic residues cysteine ​​376 (Cys376), histidine 435 (His435), and aspartic acid 458 (Asp458), which are essential for catalytic activity. Substitutions of key amino acids are shown in blue. The numbering is given for H. sapiens TGM1.

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6. Fig. 5. Electrophoretic analysis of proteins in 10% SDS-PAGE, stained with Coomassie R250. M – molecular weight marker; H. d. – clarified homogenate of H. dujardinii cell suspension. The arrow indicates the zone used for identification of H. dujardinii TGM by mass spectrometry.

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7. Fig. 6. Cell suspension 2 h after sponge dissociation. a – Control. б–г – Suspension in the presence of 1, 5, 25 mM cystamine. Scale bar – 50 µm.

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8. Fig. 7. Cell aggregates 24 h after sponge dissociation, formed in the absence (a) and presence of cystamine at a concentration of 1 mM (б); 5 mM (в) and 25 mM (г). Scale bar – 100 µm. д – Comparative analysis of aggregate area.

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9. Fig. 8. Confocal microscopy of a suspension of H. dujardinii sponge cells. Green color shows the fluorescence of cells after adding H2DCFDA to the incubation medium. White arrows show small round cells, black arrows show large cells with outgrowths.

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10. Fig. 9. Fluorescence of cell aggregates after adding H2DCFDA to the incubation medium. а,б – Control. In the presence of cystamine: в,г – 1 mM; д,е – 5 mM; g, h – 25 mM. и – Comparative analysis of fluorescence intensity.

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