Physcomitrium patens – модель для изучения эволюции белков с лектиновыми доменами у растений

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Abstract

Мох Physcomitrium (ранее Physcomitrella) patens (Hedw.) Mitt. – бессемянное и бессосудистое растение с расшифрованным геномом, представитель наиболее древних из ныне живущих таксонов наземных растений – удобная модель для изучения эволюционного развития растений. С целью изучения формирования набора и функций углевод-связывающих белков у растений в ходе эволюции проведен полногеномный скрининг генов, кодирующих белки с лектиновыми доменами, в геноме P. patens, и проанализирована их экспрессия в различных клетках и тканях мха. Выявлен 141 ген, кодирующий белки из 15 семейств, набор и число представителей которых существенно отличались от проанализированных ранее покрытосеменных растений. У P. patens некоторые из белков с лектиновыми доменами обладали специфичной доменной архитектурой, не представленной у высших семенных растений. Кластеризация генов по уровню их экспрессии в различных тканях мха выявила три паттерна экспрессии генов белков с лектиновыми доменами, из которых третий кластер, представленный в клетках с концевым типом роста (в каулонеме, хлоронеме и ризоидах мха), характеризовался наибольшим количеством активно экспрессирующихся генов. Полученные результаты подтверждают идею о раннем появлении у растений генов, кодирующих лектины, и дальнейшем расширении семейств белков с лектиновыми доменами с усложнением организации растений.

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А. Р. Агълямова

Казанский институт биохимии и биофизики Федерального исследовательского центра “Казанский научный центр Российской академии наук”

Author for correspondence.
Email: aliaglyamova@yandex.ru
Russian Federation, Казань

А. Р. Хакимова

Казанский институт биохимии и биофизики Федерального исследовательского центра “Казанский научный центр Российской академии наук”

Email: aliaglyamova@yandex.ru
Russian Federation, Казань

О. В. Горшков

Казанский институт биохимии и биофизики Федерального исследовательского центра “Казанский научный центр Российской академии наук”

Email: aliaglyamova@yandex.ru
Russian Federation, Казань

Т. А. Горшкова

Казанский институт биохимии и биофизики Федерального исследовательского центра “Казанский научный центр Российской академии наук”

Email: aliaglyamova@yandex.ru
Russian Federation, Казань

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Supplementary files

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2. Fig. 1. Schematic representation of the specific domain organization of some proteins of the hevein and LysM families from the moss P. patens (numbers of the corresponding genes are given on the left). To avoid confusion, the names of all domains are taken from the InterPro database and are therefore given in English. Designations: SP – signal peptide; Cht_bd1 (short for chitin_bind_1) – chitin-binding domain of heveins (PF00187); GH18 – domain of chitinase classes III and V (PF00704); DPBB_1 – domain of double psi beta-barrel (PF03330); LysM – lectin domain of the LysM family (PF01476); Peptidase M23 – domain of peptidase M23 (PF01551); Destabilase – domain of glutaminase (PF05497); CH – domain homologous to calponin (PF00307); TM – transmembrane domain; NT-C2 – N-terminal C2 domain (PF10358). Image created with BioRender.com.

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3. Fig. 2. Phylogenetic tree of representatives of the Jacalina family of moss P. patens, maize (Z. mays) and A. thaliana. The Robinson-Foulds distance for this tree was 0. Branching points with a support level below 90 were removed from the tree. The isolated clade of Jacalina moss is highlighted in gray.

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4. Fig. 3. Clade of the phylogenetic tree of proteins of the CrRLK1L group of the malectin-like protein family from the moss P. patens, maize (Z. mays) and A. thaliana. For ease of visualization, other clades of malectin-like proteins and malectins were removed, the full tree is presented in the Supplementary Materials, Fig. 1. The Robinson-Foulds distance for this tree was 0. Branch points with a support level below 90 were removed from the tree. Clades containing moss proteins are additionally highlighted in gray.

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5. Fig. 4. Expression of genes of the moss P. patens encoding proteins of the malectin-like and Nictaba families, as well as individual representatives of the hevein and LysM families with a specific domain organization. Their expression in different types of P. patens tissues is shown (according to [27]). For the sporophyte, the average expression value for all stages of sporophyte development is shown. The domain organization and the predicted in silico subcellular localization of proteins are shown.

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6. Fig. 5. Results of hierarchical cluster analysis and heat map of expression levels of genes encoding proteins with lectin domains in different tissue types of the moss P. patens. The heat map shows the relative expression level of each gene (rows) in each sample (rows). The expression level in clusters is presented as a Z-score transformed logarithm of expression (color scale in the upper left corner of the figure). Three identified clusters are shown on the left side as vertical rectangles (red, green, blue).

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7. Additional materials
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