Effect of Antiorthostatic Suspension on Sperm Parameters of Mus musculus Mice

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Abstract

The effect of 30-day antiorthostatic hanging of male Mus musculus mice on spermogram parameters, including the use of essential phospholipids, was studied. The concentration and motility of spermatozoa were measured, the fertilizing ability, the content of cholesterol, calcium ions and cytoskeletal proteins in spermatozoa were assessed. To assess the specificity of changes in spermatozoa, the content of cholesterol and cytoskeletal proteins was determined using buccal epithelial cells. It was shown that anti-orthostatic hanging led to a decrease in the proportion of fertilized oocytes and the absence of 2–4-cell embryos, but the use of essential phospholipids prevented these changes. The observed increase in the cholesterol content in spermatozoa, which is non-specific due to similar changes in buccal epithelial cells, in response to anti-orthostatic hanging, as well as the accumulation of calcium ions, could change the effectiveness of capacitation and cause a decrease in fertilizing capacity, which requires further research.

About the authors

K. K Gogichaeva

State Scientific Center of the Russian Federation Institute of Biomedical Problems, Russian Academy of Sciences

Email: xenitaggichaeva@gmail.com
Moscow, Russia

R. V Serebryakova

State Scientific Center of the Russian Federation Institute of Biomedical Problems, Russian Academy of Sciences

Moscow, Russia

D. I Fomina

State Scientific Center of the Russian Federation Institute of Biomedical Problems, Russian Academy of Sciences

Moscow, Russia

Yu. S Zhdankina

State Scientific Center of the Russian Federation Institute of Biomedical Problems, Russian Academy of Sciences; I.M. Sechenov First Moscow State Medical University (Sechenov University)

Moscow, Russia; Moscow, Russia

N. S Biryukov

State Scientific Center of the Russian Federation Institute of Biomedical Problems, Russian Academy of Sciences; I.M. Sechenov First Moscow State Medical University (Sechenov University)

Moscow, Russia; Moscow, Russia

O. V Kotov

State Scientific Center of the Russian Federation Institute of Biomedical Problems, Russian Academy of Sciences

Moscow, Russia

I. V Ogneva

State Scientific Center of the Russian Federation Institute of Biomedical Problems, Russian Academy of Sciences; I.M. Sechenov First Moscow State Medical University (Sechenov University); Yuri Gagarin Scientific Research and Testing Cosmonaut Training Center

Moscow, Russia; Moscow, Russia; Zvezdny Gorodok, Russia

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