Circadian Regulation of Expression of Carotenoid Metabolism Genes (PSY2, LCYE, CRTRB1, NCED1) in Leaves of Tomato Solanum lycopersicum L.

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Resumo

The circadian dynamics of the expression of key genes of carotenoid metabolism (PSY2, LCYE, CrtRB1, and NCED1) in the photosynthetic tissue of tomato Solanum lycopersicum L. (cultivar Korneevsky) plants was characterized. An in silico analysis of the gene expression pattern was carried out and a high level of their transcripts was detected in the leaf tissue. qRT-PCR analysis of gene expression was performed at six time points during the day and found the highest levels of PSY2, LCYE and NCED1 transcripts in the second half of the light phase, and CrtRB1 – at the end of the dark phase. The content and composition of carotenoids in leaf tissue in the middle of the day was determined and it was shown that the leaf accumulates 1.5 times more compounds of the ɛ/β-branch of carotenoid biosynthesis pathway than compounds of the β/β-branch.

Sobre autores

M. Filyushin

Institute of Bioengineering, Federal Research Center “Fundamentals of Biotechnology” of the Russian Academy of Sciences

Autor responsável pela correspondência
Email: michel7753@mail.ru
Rússia, Moscow

A. Shchennikova

Institute of Bioengineering, Federal Research Center “Fundamentals of Biotechnology” of the Russian Academy of Sciences

Email: michel7753@mail.ru
Rússia, Moscow

E. Kochieva

Institute of Bioengineering, Federal Research Center “Fundamentals of Biotechnology” of the Russian Academy of Sciences

Email: michel7753@mail.ru
Rússia, Moscow

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