Cytokines and inflammationCytokines and inflammationЦитокины и воспаление1684-7849Eco-Vector62707110.17816/CI627071Original Study ArticlesОригинальные исследованияResearch ArticleEffects of peptide L2 of human papillomavirus type 16 on the cytokine profile of dendritic cells in vitroВлияние пептида L2 вируса папилломы человека 16-го типа на цитокиновый профиль дендритных клеток in vitrohttps://orcid.org/0000-0001-5467-07029153-5103BoevaKsenia A.БоеваКсения Эльбертовна
Russian Federation

Junior Researcher

младший научный сотрудник

boeva_ke@niivirom.ruhttps://orcid.org/0000-0002-1259-88504161-0999MalyshkinaGalina V.МалышкинаГалина Владимировна
Russian Federation

Junior Researcher

младший научный сотрудник

malyshkina_gv@niivirom.ruhttps://orcid.org/0000-0002-3123-83599107-4118VyalykhIvan V.ВялыхИван Владимирович
Russian Federation

Cand. Sci. (Veterinary), Leading Researcher

канд. ветеринар. наук, ведущий научный сотрудник

vyalykh_iv@niivirom.ruFederal Scientific Research Institute of Viral Infections “Virome”Федеральный научно-исследовательский институт вирусных инфекций «Виром»220420241812202320224301602202402042024Copyright ©; 2023, Boeva K.A., Malyshkina G.V., Vyalykh I.V.Copyright ©; 2023, Боева К.Э., Малышкина Г.В., Вялых И.В.2023Boeva K.A., Malyshkina G.V., Vyalykh I.V.Боева К.Э., Малышкина Г.В., Вялых И.В.https://creativecommons.org/licenses/by-nc-nd/4.0https://cijournal.ru/1684-7849/article/view/627071

BACKGROUND: The human papillomavirus is an etiological factor and a biological carcinogen in tumor lesions and cancer. Currently, there are several known possible mechanisms of immune evasion of human papillomavirus. Therefore, early methods for diagnosing papillomavirus infection and effective treatments.

AIM: To analyze the cytokine profile should be established of dendritic cells in response should be determined to stimulation by peptide L2 of human papillomavirus type 16 in vitro.

METHODS: Dendritic cell cultures were obtained from the blood of donors without signs of the human papillomavirus infection in history. A culture without stimulation was used as a control. As experimental groups, cultures synthesized peptide with L2 of the human papillomavirus type 16 were used. Enzyme-linked immunosorbent assay was used to determine the levels of pro- and anti-inflammatory cytokines.

RESULTS: In comparison with spontaneous response in the control group after inoculation with peptide L2 of human papillomavirus type 16, increased IL-10, MCP-1, VEGF, IL-6, and IFN-γ concentrations and significant changes in IL-1β, IL-4, IL-8, IFN-α, and TNF-α concentrations were not detected.

CONCLUSIONS: The results confirm the ability of the human papillomavirus to evade the antiviral immune response by affecting the synthesis of several cytokines.

Обоснование. Вирус папилломы человека является этиологическим фактором и биологическим канцерогеном при опухолевых поражениях и раке. В настоящее время известно множество возможных механизмов иммунной эвазии вирусов. По-прежнему существует необходимость в расширении ранних методов диагностики инфицирования вирусом папилломы человека и поиска эффективных методов лечения.

Цель исследования — изучение цитокинового профиля дендритных клеток в ответ на стимуляцию пептидом L2 вируса папилломы человека 16-го типа in vitro.

Методы. Культуры дендритных клеток получали из образцов крови доноров, не имеющих в анамнезе признаков инфицирования вирусом папилломы человека. В качестве контроля использовали культуру без стимуляции пептидом. В качестве экспериментальных групп — культуры, в которые вносили синтезированный свободный пептид L2 вируса папилломы человека 16-го типа. Количество про- и противовоспалительных цитокинов определяли методом иммуноферментного анализа.

Результаты. После внесения пептида L2 вируса папилломы человека 16-го типа в сравнении со спонтанным ответом в контрольной группе регистрировали увеличение концентрации IL-10, МСР-1, VEGF, IL-6, IFN-γ, достоверно значимого изменения концентрации IL-1β, IL-4 и IL-8, IFN-α, TNF-α не происходило.

Заключение. Полученные результаты подтверждают способность вируса папилломы человека к уклонению от противовирусного иммунного ответа организма путём воздействия на синтез ряда цитокинов.

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